X-Rhodamin (ROX) is a red fluorescent dye that is coupled through an amino linker, hence, ROX can not be combined with other ester based linkers. ROX is qualified as reporter dye for TaqMan® probes (e.g. for RotorGene, ICycler, LightCycler® 480, RealPlex instruments) – However Texas Red® or LightCycler® Red 610 display higher intensity. Applied Biosystems uses ROX as reference dye (free ROX or the FRET system ROX--FL – both is available at TIB MOLBIOL). ROX can be combined with BHQ-2, BHQ-3 and BBQ (BlackBerry) quenchers. We recommend BBQ.

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