HEX (Hexachlorfluorescein)

HEX is used as a reporter dye for TaqMan® probes and sequencing (ABI). HEX is already relatively unstable in the production. HEX labeled primers are very hydrophobe, affecting PCR efficiency. Thus, HEX is being substituted by different dyes. Applied Biosystems uses VIC, we recommend Yakima Yellow (YAK), JOE, R6G or Cy3.

HEX can be combined with TAMRA, Dabcyl, BHQ-1, BHQ-2 and BBQ (BlackBerry) Quenchers. We recommend BBQ.

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