TaqMan® Probes

TaqMan® probes are oligonucleotides, which consist of a reporter dye and a quencher. They are blocked against an extension on their 3’-terminal.

During the PCR-reaction, they are hydrolyzed resulting in a release of the fluorescent dye (5-nuclease-assay).

TaqMan® probes are used for Real-Time detection of PCR products (like Molecular Beacons and LightCycler® HybProbe).

All products are submitted to several purification steps, including three HPLC-passages, to ensure a complete labeling. Minimal amounts of unquenched molecules would lead to a high intrinsic initial fluorescence (high background).

Scales

Set (guaranteed) delivery amounts in OD260 (1 to 50 OD) or Nanomol (3 to 150 nmol) and evaluation scale 0,1 OD.

Reporter

We offer a wide spectrum of fluorescent dyes from UV to red.

Quencher

TAMRA (Tetramethylrhodamin) is fluorescent itself (internal through thymidin or 3'-terminal).

'Dark Quencher' do not emit any light and do not interfere with the measurement:

Dabcyl (DiAzoBenzyl = Methylorange), BHQ1, BHQ2, BHQ3, BBQ.

Carry over to OligoShop