Primer HPLC

The oligonucleotide synthesis has an efficiency of more than 99% per coupling step.

Chains that failed to be extended during the coupling step are acetylated to prevent sequences with deletions. This, so called capping step, is not 100% efficient, resulting in very low numbers of deletion carrying products. Overall, the average efficiency of solid phase oligonucleotide synthesis is about 99%. We recommend the HPLC-purification for longer primers (>40nt). The hydrophobic final trityl-protection group is not removed at the final synthesis step. After the deprotection the product will be purified through a reversed phase HPLC column. This method allows the removal of shortened (n-1) products. Other defective sequences, especially deletions, insertions and substitutions will not be disposed through this method.

The anion exchanger purification (FPLC) enables the purification of oligonucleotides by their length. This procedure eliminates defective sequences.

A combination of both methods guarantees the best purity.

We do not offer the polyacryl amid gel (PAGE) purification, since we do not want our employees to be constantly exposed to the neurotoxin acryl amid. Furthermore, this method can only be applied to small amounts.

Carry over to OligoShop