Primer custom synthesis
Primers are unmodified oligonucleotides with a typical length of 17 to 40 bases; the maximum viable length would be around 200 nucleotides. We use a gel filtration (GSF) for this category of primers; longer sequences (>40nt) are preferably purified by HPLC.
We offer synthesis with GSF purification in different scales:
|
Synthesis scale |
Qty in OD |
Qty in nmol |
PCR Reactions |
|
0,02 µmol |
2-3 |
10-15 nmol |
1.000-1.500 |
|
0,20 µmol |
20-30 |
100-150 nmol |
> 10.000 |
|
2,00 µmol |
100-120 |
500 nmol |
> 50.000 |
|
20,0 µmol |
500-600 |
5.000 nmol |
> 500.000 |
The molar yield decreases with chain length. The above mentioned values are classified for 17 to 30 mer sequences. The quantity of viable PCR reaction is based on the use of 10 pmol primer in 20 µl volume (0,5 µM).
Top