Primer custom synthesis

Primers are unmodified oligonucleotides with a typical length of 17 to 40 bases; the maximum viable length would be around 200 nucleotides. We use a gel filtration (GSF) for this category of primers; longer sequences (>40nt) are preferably purified by HPLC.

We offer synthesis with GSF purification in different scales:

Synthesis scale

Qty in OD

Qty in nmol

PCR Reactions

0,02 µmol

2-3

10-15 nmol

1.000-1.500

0,20 µmol

20-30

100-150 nmol

> 10.000

2,00 µmol

100-120

500 nmol

> 50.000

20,0 µmol

500-600

5.000 nmol

> 500.000

The molar yield decreases with chain length. The above mentioned values are classified for 17 to 30 mer sequences. The quantity of viable PCR reaction is based on the use of 10 pmol primer in 20 µl volume (0,5 µM).

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