Working Concentration (Labeled Primer, Probe, Reverse Primer)

Opening Vials Containing Lyophilized Products:

Spin down before opening. The pellet may have relocated during shipment.

Hybridization Probes for the LightCycler® Instruments

HybProbes are delivered in fixed molar amounts of 300 pmol, 1 nmol, 3 nmol, or 30 nmol.

Do not freeze lyophilized probes. Products are stable at room temperature. Protect from light exposure.

Usage with the LightCycler® Instruments

When using an internal LC Red 640 labeled primer instead of a probe pair, the fluorescein probe will be located on the opposite strand. The concentration of the labeled primer is as for an amplification primer:

Asymmetric (internal labeled primer, probe)	Concentration in the reaction	Molar amounts in Master Mix		per reaction	Vol. per reaction
		32 samples 640 µl	10 samples 200 µl	1 sample 20 µl	Volume of stock/reaction
Primer iLC	0.50 µM	320 pmol	100 pmol	10 pmol	1 µl/10 µM
Probe 3FL	0.10 µM	64 pmol	20 pmol	2 pmol	1 µl/3 µM
2. Primer	0.20 µM	128 pmol	40 pmol	4 pmol	0.4 µl/10 µM