Working Concentration (Labeled Primer, Probe, Reverse Primer)
Opening Vials Containing Lyophilized Products:
Spin down before opening. The pellet may have relocated during shipment.
Hybridization Probes for the LightCycler® Instruments
HybProbes are delivered in fixed molar amounts of 300 pmol, 1 nmol, 3 nmol, or 30 nmol.
Do not freeze lyophilized probes. Products are stable at room temperature. Protect from light exposure.
Usage with the LightCycler® Instruments
When using an internal LC Red 640 labeled primer instead of a probe pair, the fluorescein probe will be located on the opposite strand. The concentration of the labeled primer is as for an amplification primer:
Asymmetric (internal labeled primer, probe) |
Concentration in the reaction |
Molar amounts in Master Mix |
|
per reaction |
Vol. per reaction |
|
|
32 samples 640 µl |
10 samples 200 µl |
1 sample 20 µl |
Volume of stock/reaction |
Primer iLC |
0.50 µM |
320 pmol |
100 pmol |
10 pmol |
1 µl/10 µM |
Probe 3FL |
0.10 µM |
64 pmol |
20 pmol |
2 pmol |
1 µl/3 µM |
2. Primer |
0.20 µM |
128 pmol |
40 pmol |
4 pmol |
0.4 µl/10 µM |