Preparing of Stocks Solutions, Handling and Storage

Opening Vials Containing Lyophilized Products:

Spin down before opening. The pellet might be attached to the lid.
 

Hybridization Probes for the LightCycler®

HybProbes are delivered in fixed molar amounts of 300 pmol, 1 nmol, 3 nmol, 6 nmol or 30 nmol.

Do not freeze lyophilized probes. Products are stable at room temperature. Store dark.

Usage with the LightCycler®

Preparing of Stock Solution 3 µM for Probes and 10 µM Primer

Amount

300 pmol            

1 nmol                

3 nmol   

30 nmol     

HybProbe 3FL or 5LC

100µl

333 µl

1000µl

1000µl

 

3µM

3µM

3 µM

30µM 1:10

Concentration

3µM

3µM

3µM

3µM

Primer iLC

1 nmol

3 nmol

30 nmol

 

100µl

300µl

300µl 1:10

Concentration     

10µM

10µM

10µM

PCR-Primer

1,0 OD260 ~ 5 nmol

10-15 nmol

500µl

1000-1500µl

Dilution of dissolved primers

Samples (for a 10µM solution)

14µM 4µl aq + 10µl oligo
125µM 115µl aq + 10µl oligo

Storage

Store lyophilized products at room temperature or refrigerated. Do not freeze lyophilized products!

Store dissolved probes refrigerated (4°C) or frozen (-20°C). Avoid multiple cycles of freezing and thawing. We suggest to prepare aliquots store frozen and keeping one aliquot refrigerated for daily work. Please read the product description carefully for dilution instructions. Add 100 µl/1 nmol for a 10 µM Stock solution. For dissolved products > 10 µM take a volume = concentration in µM minus 10 µl water and ad 10 µl oligo to prepare a 10 µM solution.