Amounts and available modifications

A set of hybridization probes consists of a pair of oligonucleotide probes which can hybridize neighbored on a DNA template, one labeled with fluorescein at its 3'-terminus [3FL] and the other labeled at its 5'-end with LightCycler® Red 640 (LC640) or LC705 [5LC]. The free 3'-hydroxy group will usually be blocked with a phosphate against polymerase extension. There should be a distance between both probes of one to four bases (4 to 25 Å distance), to leave space for the fluorophores.

Code

Product

Amount 

Tests 

Label

17-0640-01

Custom Synthesis 5'-LC640,
3'-Phosphat

1 nmol

300

5LC 1,0

17-0640-03

Custom Synthesis 5'-LC640,
3'-Phosphat

3 nmol

1.000

5LC 3,0

17-0640-06

Custom Synthesis 5'-LC640,
3'-Phosphat

2 x 3 nmol

2.000

5LC 2x3

17-0640-30

Custom Synthesis 5'-LC640,
3'-Phosphat

30 nmol

10.000

5LC 30

17-0640-33

Custom Synthesis 5'-LC640,
3'-Phosphat

10 x 3 nmol

10.000

5LC 30

17-0644-01

Custom Synthesis int. dT-
C2-LC640, 3'-OH

1 nmol

300

iLC 1,0

17-0644-03

Custom Synthesis int. dT-
C2-LC640, 3'-OH

3 nmol

1.000

iLC 3,0

17-0644-30

Custom Synthesis int. dT-
C2-LC640, 3'-OH

30 nmol

10.000

iLC 30

17-0705-01

Custom Synthesis 5'-LC705,
3'-Phosphat

1 nmol

300

5LC 1,0

17-0705-03

Custom Synthesis 5'-LC705,
3'-Phosphat

3 nmol

31.000

5LC 3,0

17-0705-06

Custom Synthesis 5'-LC705,
3'-Phosphat

2 x 3 nmol

2.000

5LC 2x3

17-0705-30

Custom Synthesis 5'-LC705,
3'-Phosphat

30 nmol

10.000

5LC 30

17-0705-33

Custom Synthesis 5'-LC705, 3'-Phosphat

10 x 3 nmol

10.000

5LC 30

17-2121-01

Custom Synthesis 3'-Fluorescein

1 nmol

300

3FL 1,0

17-2121-03

Custom Synthesis 3'-Fluorescein

3 nmol

1.000

3FL 3,0

17-2121-06

Custom Synthesis 3'-Fluorescein

2 x 3 nmol

2.000

3FL 2x3

17-2121-30

Custom Synthesis 3'-Fluorescein

30 nmol

10.000

3FL 30

17-2121-33

Custom Synthesis 3'-Fluorescein

10 x 3 nmol

10.000

3FL 30

17-2640-00

HybProbe 640 Set LC640/3FL

per 300 pmol

100

0,3

17-2705-00

HybProbe 705 Set LC705/3FL

per 300 pmol

100

0,3

Delivered amounts and assays

HybProbes are available in the following product amounts: 1 nmol, 3 nmol and 30 nmol. The 300 pmol scale is for evaluation of the system and is only available as set. Other scales on request. The prices include the synthesis of the oligonucleotides (30 bases). All products are purified by a multistep HPLC procedure and desalting. Delivery lyophilized. Numbers of tests were calculated assuming the use of 3 pmol per assay. In routine this amount may vary between 2 pmol (0,1 µM) and 10 pmol (0,5 µM) probe per assay.

300 pmol

1 nmol

3 nmol

2 x 3 nmol

30 nmol

100 Tests

300 Tests

1.000 Tests

2.000 Tests

10.000 Tests