DNA Methylation

Methylation of genomic DNA is essential for the development and is associated with a number of key processes such as imprinting, X-chromosome inactivation, suppression of repetitive elements, control of expression and also in carcinogenesis.

DNA methylation involves the addition of a methyl group to DNA - most important is the methylation at position 5 in the cytosine pyrimidine ring (mC) - which is found especially in CpG sequence motifs. In mammals 70-90% of the CpG sequences are methylated. Some unmethylated CpG's are located in the regulatory 5'-region of genes which can be in case of cancer hypermethylated, thus reducing the expression of the respective gene.

Methylation protects DNA during bisulfate treatment, allowing to analyze the methylation status of a given sequence.

Epigenomics AG in Berlin has focused on the analysis of methylated genes.

We offer one hybridization probe based LightMix® kit for GSTP1 and a control kit which have been developed by Epigenomics.