Legionella pneumophila (16S RNA)

Legionella pneumophila is a thin, faintly staining gram-negative non-spore forming bacteria and the most common species of the family of Legionellaceae. Legionella pneumophila, which is responsible of the vast majority of human infections caused by Legionellaceae, is widespread in the environment and is associated almost exclusively with surface and potable water or moist environment.

Many Legionella infections are subclinical, while pneumonia is the most frequent manifestation: being responsible of the 5% of the pneumonia cases. The onset is usually abrupt with high fever, malaise, myalgias, headache and a non productive cough. Only in very rare cases Legionella infections can target districts other than lung: pericarditis, myocarditis, endocarditis, pancreatitis, and hepatic abscesses have been reported.

The 16S RNA PCR primers amplify several species of Legionella; the hybridization probes are specific for L. pneumophila. The LightMix® Kit for Legionella 16S has a detection limit of 10 copies (in an exemplary system, using a cloned target as reference). The linear measuring range of the assay is 102 to 106 copies. PCR results are obtained within 1 hour.

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