Influenza A H1N1/2009 pandemic (discontinued)

RT-PCR based detection of Influenza A is based on the conserved matrix protein gene (MP). Additional tests can be used for the identification of the hemagglutinin and the neuraminidase gene types. The actual H1N1 swine virus has a NA gene which is similar to H5N1 but a very specific HA gene, which can be used for its identification. An test for NA is thus not essential.

A network of German virologist from Bonn, Freiburg and Marburg has developed a new 5-nuclease (TaqMan) assay for the specific detection of the swine virus H1 gene.

Detection of Influenza A(H1N1)v virus by real-time RT-PCR. Panning et al., Eurosurveillance Vol. 14 (2009) www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19329

We have combined the primers and probes for the detection of the Matrix Protein gene (MP or M2) published by Ward et al., 2004 (2007 WHO recommendation for bird flu H5N1 detection) and this novel assay for the H1 gene in one test package, containing each 100 reactions of pre-mixed primers and probes with FAM label, including a positive control for both.

Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. Ward et al. J Clin Virol. 2004 Mar;29(3):179-88

This kit has been distributed since end of April 2009 as an emergency solution for the detection of the novel InfA H1N1/2009 virus and has been discontinued when the first licensed PCR kits became available.