Human Herpes Virus 6 (HHV-6)

HHV-6 (Human Herpesvirus 6) infects human white blood cells, specifically T lymphocytes. More than 95% of the world's population is positive for antibodies to HHV-6. Most infections occure in infants many of them displaying mild or no symptoms. Roseola is the main disease caused by HHV-6, a small number of infected infants can develop serious disease including bone marrow infection and brain infection. The factors that lead to reactivation in people with intact and functioning immune systems are unclear and probably include genetic and environmental causes. Most instances of reactivation will not result in chronic, active infection as the normal immune system will suppress the reactivated virus and return it to a latent state. However, reactivation of HHV-6 in normal adults has been associated with a mononucleosis syndrome, autoimmune disorders, and nervous system diseases (e.g Multiple Sclerosis).

The LightMix® Kit HHV-6 is based on the amplification of the antigenic virion protein 101K (U11) gene and provides a fast, easy and accurate system to identify and quantify this target in a nucleic acid extract. A control amplification reaction acts as internal control (IC). This kit has been tested on the LightCycler® 1.x / 2.0 / 480 II Instruments with Roche Diagnostics ‘LightCycler® FastStart DNA Master HybProbe’.

References:

Use of Real-Time PCR to monitor human herpesvirus 6 reactivation after allogeneic bone marrow transplantation Ohyyashiki, J.H. et al., Int.J.Mol.Medicine 6 (2000) 427-432

Additional reagents and items required

Color Compensation Kit 530/640/690

40-0318-99

LightCycler® FastStart DNA Master HybProbe

03 003 248 001

Capillaries (20 µl) (LC1.x / 2.0 Instruments)

04 929 292 001

LC 480 Multiwell Plate 384, white

04 729 749 001

Carry over to OligoShop