Transcription is highly regulated; altered transcript levels can be the consequence of a disease. The study of expression is a powerful tool in medical research. Measurements are normalized against the overall amount of mRNA using the expression level of reference or housekeeping genes.

Human glucose-6-phosphate dehydrogenase (human G6PDH) represents a reference gene, it is expressed in all cells, where its product plays a key role in the pentose phosphate pathway.

Accurate and rapid analysis of residual disease in patients with CML using specific fluorescent hybridization probes for real time quantitative RT-PCR. Emig M. et al., Leukemia (1999) 13, 1825-1832

A 194 bp fragment of the human G6PDH cDNA is amplified with specific primers. The resulting PCR product is detected with LightCycler® Red 640 labeled hybridization probes (detected in channel 640).

The supplied standard row allows to determine the linear range of the reaction and to estimate the quantity of the target sequence in unknown samples.

Carry over to OligoShop