EHEC toxin genes stx1/2

Escherichia coli are bacteria commonly found in the gut of humans. While most strains of E. coli are harmless there are some diarrheagenic strains causing severe disease. Transmission to humans is primarily foodborne through consumption of contaminated raw or undercooked foods.

The enterohaemorrhagic E. coli (EHEC) produce the Attaching and Effacing protein (Eae) and the Shiga-like toxins stx1 and/or stx2 which are closely related to toxins from Shigella dysenteriae.

The LightMix® Kit EHEC is based on the publication from Grys et al., 2009 and detects the toxin genes stx1 and 2 and differentiates them by their specific melting temperatures.

The recent (May 2011) EHEC isolates contain only the stx2 toxin gene and miss the eae gene.

Rapid and Sensitive Detection of Shiga Toxin-Producing Escherichia coli from Nonenriched Stool Specimens by Real-Time PCR in Comparison to Enzyme Immunoassay and Culture, Grys et al., JCM, 2008–2012 (2009)

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