Epstein-Barr Virus (EBV)

The Epstein-Barr virus (EBV or human herpesvirus 4, HHV-4) of the family Herpesvirus is one of the most common human viruses. The virus occurs worldwide. Children infected with EBV, often show no symptoms or symptoms are indistinguishable from other mild, brief illnesses.

When infection with EBV occurs during adulthood, it causes infectious mononucleosis with several symptoms (fever, sore throat, swollen lymph glands, rarely heart problems or involvement of the central nervous system) which last for some weeks but is seldom fatal. Reactivation of the virus after a period of illness usually occurs without symptoms.

There is no specific treatment for infectious mononucleosis, other than treating the symptoms but nevertheless identification of EBV may be needed for verification and to differentiate EBV infections from a mononucleosis-like illness induced by cytomegalovirus, adenovirus, or Toxoplasma gondii.

In very rare cases EBV carriers may develop Burkitt's lymphoma and nasopharyngeal carcinoma. EBV appears to play an important role in these malignancies, but is probably not the sole cause of the disease.

The LightMix® Kit EBV is based on the amplification of the EBNA-1 gene and provides a fast, easy and accurate system to identify and quantify this target in a nucleic acid extract. A control amplification reaction acts as internal control (IC). This kit has been tested on the LightCycler® 1.x and 2.0 Instruments with Roche Diagnostics ‘LightCycler® FastStart DNA Master HybProbe’.

References

Detection of HSV-1/-2, VZV, EBV and CMV by a set of LightCycler PCRs Performed in Parallel within a Single Run. Stöcher, M., Leb, V. Bosic, M.,Kessler, H. Halwachs-Baumann, G., Landt, O., Stekel, H. and Berg, J., J Clin Virol. 26 (2003) 85-93

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