LightMix® - Universal Color Compensation Set

For in vitro diagnostic procedures.

Different channels for hybridization probes are available in the various LightCycler® Instruments :

Channel

Instrument

530

F1

610

640

F2

>660

670

705

F3

690

F3

LightCycler 1.2/5

X

 

X

 

 

X

X

LightCycler 2.0

X

X

X

 

X

X

X

LightCycler 480

X

X

X

 

X

 

 

LightCycler 480 II

X

X

X

X

 

 

X

As from the table above, channels 530 and 640 are present in all the instruments, making this labeling combination appropriate for duplex kits and suitable for transfer among instruments.

We have introduced the new dye LC690 (with a wide emission band) which can be read either in the 705 or F3 channel of the capillary based LightCycler® Instruments or in the >660 channel of the LightCycler® 480 II Instrument (640/690).

This kit provides standards to create a ColorCompensation file to run LightCycler® assays with the dye combinations 530/640 (genotyping combining SimpleProbe® and hybridization probes) and 640/690 (detection/quantification) using the LightCycler® Instruments 1.2, 1.5, 2.0 and 480 II (480 for 530/640 only).

09/2009 New version for 530/640 and 640/690 LightMix® Kits.

Carry over to OligoShop