Bordetella pertussis and parapertussis

Pertussis (whooping cough) is a fatal respiratory disease in children caused by a bacterial infection with Bordetella pertussis - the mortality rate is 0.5% in infants under six months 1. A milder form of the disease is caused by B. parapertussis while B. holmesii and B. bronchiseptica are rare in humans. Diagnosis with immune fluorescence is limited to the first three weeks of the infection while serology and PCR based methods are reported be useful for an extended period of time. Simultaneous detection of B. pertussis and parapertussis using insertion sequences (IS) and the LightCycler® instrument has been published by Reischl et al., 2001.

The kit is based on the amplifcation of a fragment from the IS481 region for detection of B. pertussis, using hybridization probes with an LC640 label, and the IS1001 region for B. parapertussis, using probes with an LC690 label. The internal control (IC) is detected with very short probes with LC640 label, resulting in a specific melting peak at 49.5°C only.

This kit can be used with LightCycler® 1.2 / 1.5 / 2.0 and 480 II instruments.

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