Transcription is highly regulated; altered transcript levels can be a consequence of a disease. The study of expression is a powerful tool in medical research. Measurements are normalized against the overall amount of mRNA using the expression level of reference or housekeeping genes.

Human Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) is expressed ubiquitously and represents a reference gene. The biological function of the protooncogene ABL1 tyrosine-specific protein kinase is to play a key role in cell signaling processes (stress response, cell division, cell adhesion, cell differentiation). In Europe the ABL1 gene was chosen as the preferred reference for the quantification of fusion gene transcripts in leukemic residual disease detection

1 Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program. Gabert J. et al. Leukemia 17:2318-2357 (2003).

2 Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using ‘real-time’ quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) – a Europe Against Cancer program. Beillard E. et al. Leukemia 17:2474-2486 (2003).

A 137 bp fragment of the human ABL1 cDNA is amplified with specific primers. The resulting PCR fragment is analyzed with hybridization probes labeled with LightCycler® Red 640 (detected in channel 640).

The supplied standard row allows to determine the linear range of the reaction and to estimate the quantity of the target sequence in unknown samples.

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