Preparing of Stocks Solutions, Handling and Storage

Opening Vials Containing Lyophilized Products:

Spin down before opening. The pellet might be attached to the lid.
 

Hybridization Probes for the LightCycler®

HybProbes are delivered in fixed molar amounts of 300 pmol, 1 nmol, 3 nmol, 6 nmol or 30 nmol.

Do not freeze lyophilized probes. Products are stable at room temperature. Store dark.

Usage with the LightCycler®

Preparing of Stock Solution 3 µM for Probes and 10 µM Primer

Amount	300 pmol	1 nmol	3 nmol	30 nmol
HybProbe 3FL or 5LC	100µl	333 µl	1000µl	1000µl
	3µM	3µM	3 µM	30µM 1:10
Concentration	3µM	3µM	3µM	3µM
Primer iLC		1 nmol	3 nmol	30 nmol
		100µl	300µl	300µl 1:10
Concentration		10µM	10µM	10µM

PCR-Primer	1,0 OD260 ~ 5 nmol	10-15 nmol
	500µl	1000-1500µl
Dilution of dissolved primers	Samples (for a 10µM solution)	14µM 4µl aq + 10µl oligo 125µM 115µl aq + 10µl oligo

Storage

Store lyophilized products at room temperature or refrigerated. Do not freeze lyophilized products!

Store dissolved probes refrigerated (4°C) or frozen (-20°C). Avoid multiple cycles of freezing and thawing. We suggest to prepare aliquots store frozen and keeping one aliquot refrigerated for daily work. Please read the product description carefully for dilution instructions. Add 100 µl/1 nmol for a 10 µM Stock solution. For dissolved products > 10 µM take a volume = concentration in µM minus 10 µl water and ad 10 µl oligo to prepare a 10 µM solution.