The Lightcycler from Roche Diagnostics is the fastest thermocycler with online monitoring of DNA amplification. The system works with heated air, allowing rapid ramping – 30 cycles can be performed within 20 minutes.
The LightCycler® detects fluorescence in three seperated channels. For quantification the growing PCR product is monitored using a DNA-binding dye, or using TaqMan or hybridization probes.
Some of the mayor andvantages of the Real-time detection are the direct quantification of the amplicons and, because avoiding post – PCR handling, a minimal contamination risk with PCR products.
The LightCycler® melting curve analysis allows the accurate detection of single base mismatches. This unique feature enables rapid analysis of polymorphisms in genetics, clinical chemistry, forensic analysis, pharmacology and other research fields.
When using exteral standards of known copy numbers, the LightCycler® will enable the exact determination of template amount. When working with plasmid standards we recommend the use of dUracil-containing preparations to minimize the contamination risk. External custom standards are available from GenExpress GmbH, Berlin.