Since 1990, TIB MOLBIOL has been offering custom synthesis of oligonucleotides to a worldwide growing number of customers. As one of the first suppliers of synthetic DNA on the European market, we have since then set standards of quality and service. Our core competence still lies in the synthesis of nucleic acids and analoga; we have transferred a selection of our other Molecular Biology custom services to newly established companies. Our GenExpress laboratory offers cloning and expression services. Today, our oligonucleotide production facilities in Germany, Italy and Poland offer a broad spectrum of products and services. Our customer's application fields vary and range from life science research and environmental analysis to medical diagnostics over product quality assessment. Oligonucleotides are employed as primers in the PCR, for target quantification, for DNA sequencing, as hybridization probes and as AntiSense reagents or therapeutics.The individual requirements of the users have kept a relatively simple chemical molecule in an exceptional position. Particularly PCR has revolutionized laboratories of molecular biology giving way to a vast number of applications and possibilities. The knowledge of these applications, a contribution to their debate, an estimation of their potential, to recommend or suggest them, or even to try them out, means a continuous confrontation with new techniques. The awareness of the individuality of the product is thus a central feature of custom synthesis. We have therefore focused on the assistance of our customers in developing and designing the appropriate molecules. 9. Concurrently, the demands on our products have changed leading to new concepts which we regularly transform in new product profiles.
TIB MOLBIOL is committed to high quality - the demands for quality apply not only to the product itself but furthermore it includes diligent and swift production, a service void of annoyance and consideration of environmental care. We believe that the basic key for a controlled process is our staff of skilled and experienced professionals. Our synthesis laboratories are air-conditioned under lowest air humidity, ensuring constant production conditions throughout the year. Finally, the continuous monitoring of all processes, our strict quality management system, the exclusive use of certified reagents and a final quality check guarantee the highest possible quality and consistency of each product, a guarantee for reproducible results.
Fluorescence has changed the possibilities for the detection of molecules and molecular interaction widely, not only reaching the sensitivity of radioactivity but also allowing new insights, for example time-resolved data and parallel / multiplex measurements. Fluorescence is used to measure enzyme kinetics, for the analysis of DNA sequences, detection on arrays, in-situ hybridization and chromosome painting, and the number of applications is still growing. Today many advanced applications are based on energy transfer between two fluorophores, or, on quenching of a fluorophore, both depending on the distance between the two interacting molecules, hence working as a sensitive monitor of their vicinity, producing signals, without washing steps, the use of developer reagents or enzyme substrates.
The newly introduced Real-time PCR monitoring is based on the FRET energy transfer. It is our pleasure to offer the custom synthesis of hybridization probes to be used with the new LightCycler®. For the first steps with this instrument we are happy to lend our assistance in the design of suitable probes, either for product quantification or nucleotide polymorphism detection. On request we check your system, we give you our suggestions or we design HybProbe assays based on your established PCR primers. We use different programs to analyze the melting temperature, thermodynamic stability, hybridization kinetics, sequence structure, false priming and search Genbank entries. To ensure an efficient support you should provide us with the sequences of the primers you are using, if available the Genbank accession number of the target sequence or the target sequence in a machine readable format (e-mail, txt or rtf file preferred). Especially in the case of multiple Genbank entries, known variants, subtypes and strains we need further information regarding the requirements of your assay, e.g. detection of families, selective detection, sub-typing, variant exclusion, multiplex testing and/or other. All information provided will be treated confidentially. Naturally this comprises all sequences as well as the projects and laboratories involved. For some applications we have our own systems or we may know already published assays. Please inquire The check of your HybProbe design is a service of TIB MOLBIOL, free of charge. In the case of a complete design of your assay we appreciate the acknowledgement of our contribution in the publication. Custom design is also available as a chargeable service. Collaboration projects are welcomed.